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KMID : 0900919990230020113
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Korean journal of Animal Reproduction
1999 Volume.23 No. 2 p.113 ~ p.118
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Studies on the Fertilization Rates using Intracytoplasmic Sperm Injection with In Vitro Matured Porcine Oocytes
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Kim Sang-Keun
Kim Min-Soo
Nam Yoon-Yi
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Abstract
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This study was carried out to investigate on the improvement of fertilizing ability of in vitro matured oocytes from sperm density and motility by intracytoplasmic sperm injection(ICSI) into the porcine oocytes. 1. The in vitro fertilization and cleavage rates of oocytes from 1.0, 2.0, 3.0, 5.0 (times10^{6} mell) sperm concentration by IVF and ICSI of porcine oocytes were 46.7%~75.0%, 60.0%~85.7% and 10.6%~25.0%, 20.0%~64.3%, respectively. 2. The in vitro fertilization and cleavage rates of oocytes from 20, 40, 60, 80% of sperm mortilty by IVF and ICSI of porcine oocytes were 46.4%~71.4%, 67.9%~85.7% and 7.1%~21.4%, 28.6%~60.7%, respectively. 3. The in vitro fertilization and developmental rates of oocytes by IVF and ICSI methods were 55.6%~60.0%, 77.8%~80.0% and 17.8%~24.0%, 42.2%~56.0%, respectively. This ICSI method was improved high fertilization rates of porcine oocytes.
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KEYWORD
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Porcine oocytes, IVF, ICSI, Fertilization, Cleavage rates
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